anti myosin heavy chain mhc antibody  (Developmental Studies Hybridoma Bank)

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Comparison of mRNA expression levels of SM-MHC (A), α-SMA (B), SM-calponin (C), S100A4 (D), F4/80 (E), IL-6 (F), MCP-1 (G), Ccl5 (H), TGF-β (I), and Col1a1 (J) between SKO mice with AAA and DKO mice without aneurysm formation. Real-time PCR shows no significant differences in the mRNA expression levels of α-SMA , SM-MHC , or SM-calponin between the two groups. The mRNA expression levels of S100A4 , F4/80 , MCP-1 , Ccl5 , TGF-β and Col1a1 were significantly lower in the DKO group, without aneurysm formation, than in the SKO group, with AAA. Each data point represents an individual mouse (biological replicate). The numbers of analyzed samples were as follows: SM-MHC (SKO n = 8, DKO n = 7), α-SMA (SKO n = 7, DKO n = 7), SM-calponin (SKO n = 8, DKO n = 7), S100A4 (SKO n = 8, DKO n = 7), F4/80 (SKO n = 6, DKO n = 7), IL-6 (SKO n = 7, DKO n = 7), MCP-1 (SKO n = 8, DKO n = 7), Ccl5 (SKO n = 7, DKO n = 7), TGF-β (SKO n = 8, DKO n = 7), and Col1a1 (SKO n = 7, DKO n = 7). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant in Kruskal-Wallis test.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Quantitative histopathological and immunohistochemical analysis of aortic tissues. Representative images of Alcian blue staining (A, B), SM-MHC immunostaining (D, E), Mac-2 immunostaining (G, H), S100A4 immunostaining (J, K), MCP-1 immunostaining (M, N), and Ccl5 immunostaining (P, Q) in the SKO and DKO groups. Representative images from the SKO and DKO groups are shown in the upper and middle rows, respectively, and the corresponding quantitative analyses are shown in the bottom row. The corresponding quantitative analyses are shown in panels C, F, I, L, O, and R, respectively. The stained areas were quantified using ImageJ. For Alcian blue, SM-MHC, S100A4, MCP-1, and Ccl5 staining, the positive staining areas were normalized to the medial area of the aortic wall. For Mac-2 staining, the positive area was normalized to the combined intimal and medial area because macrophage infiltration was frequently observed in both layers. Each dot represents the value obtained from a single analyzed section. Sections containing advanced aneurysmal lesions were excluded to avoid secondary changes, and samples with inadequate tissue orientation were also excluded from the analysis. The numbers of analyzed sections were as follows: Alcian blue staining (SKO n = 21, DKO n = 39), SM-MHC staining (SKO n = 20, DKO n = 35), Mac-2 staining (SKO n = 29, DKO n = 37), S100A4 staining (SKO n = 15, DKO n = 15), MCP-1 staining (SKO n = 14, DKO n = 16), and Ccl5 staining (SKO n = 14, DKO n = 16). Statistical comparisons between groups were performed using the Kruskal–Wallis test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Immunohistochemical staining, Staining, Immunostaining